In Vitro Effects of Some Bio-indoles on the Transmembrane Potential of Jurkat E 6.1 Limphoblasts

In this paper we proposed to research the biochemical mechanisms of the biocompounds with indolic structure, by investigating their biochemical effects on one very important physico-chemical parameter of the cell membrane, namely the (trans)membrane potential. The aim of the study was the assessment of the membrane depolarizationrepolarization processes induced by some bio-indoles, namely melatonin, serotonin, and tryptophan. To this effect we performed a spectrofluorimetric study using as experimental model the Jurkat E 6.1 lymphoblast cell line and the voltage-sensitive probe bis-(1,3 dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)). The obtained results showed that all the studied bio-indoles have a cell membrane hyperpolarization effect.